Mass Spectrometry as a majorly used instrument in proteomics

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Mass spectrometry is an analytical tool useful for measuring the mass-to-charge ratio (m/z) of one or more molecules present in a sample. These measurements can often be used to calculate the exact molecular weight of the sample components as well.Mass spectrometry (MS) is an analytical technique that separates ionized particles such as atoms, molecules, and clusters by using differences in the ratios of their charges to their respective masses (mass/charge; m/z), and can be used to determine the molecular weight of the particles.ToF, magnetic sector and quadrupole mass spectrometers are all commonly used in SIMS instrumentation. The Rapid Toxic Screen used by the CDC, or Centers for Disease Control, is an example of mass spectrometry, and it can detect chemical agents in blood and urine that are often used in terrorism, like cyanides or deadly nerve toxins.Specific applications of mass spectrometry include drug testing and discovery, food contamination detection, pesticide residue analysis, isotope ratio determination, protein identification, and carbon dating. In a mass spectrometry experiment, the process sequence of analysis works in five stages, including sample introduction, analyte ionization, mass analysis, ion detection, and data processing. The MS/MS has three major advantages: The capacity to study numerous molecules regardless of whether they are from the same structural family or not; The capacity to highlight the specific metabolites of a disease; It's an automated technique offering the possibility of large-scale analysis. A spectrometer (/spɛkˈtrɒmɪtər/) is a scientific instrument used to separate and measure the spectral components of physical phenomena. A spectrometer is a broad term often used to describe an instrument that measures a continuous variable of a phenomenon whose spectral components are mixed in some way. In MS, molecules are bombarded with high-energy electron beams, in NMR with radio waves, and in IR with thermal energy. UV-Vis spectroscopy exposes molecules to ultraviolet light. Or with a visible beam, make it brighter.

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